diff --git a/configure.ac b/configure.ac
index 7f7967fdee..2147f2d279 100644
--- a/configure.ac
+++ b/configure.ac
@@ -61,7 +61,6 @@ PKG_CHECK_MODULES(PYGTK, pygtk-2.0 >= $PYGTK_REQ)
 AC_SUBST(PYGTK_CFLAGS)
 
 PKG_CHECK_MODULES(PYGTK, pygtk-2.0 >= 2.3.92, ,HAVE_OLD_PYGTK="yes")
-AC_SUBST(PYGTK_CFLAGS)
 if test "x$HAVE_OLD_PYGTK" = "xyes"; then
   AC_DEFINE_UNQUOTED(HAVE_OLD_PYGTK, 1, [Defined if we have an old version of PyGTK])
 fi